The city-university partnership Cyride (http://www.cyride.com) provides bus service to most of the city. Fare is $1 $0.50 for children/seniors, with monthly passes available for $35.
Lincoln Way is the main east-west thoroughfare of the city, running from just south of downtown west to Campustown and along the southern edge of the central campus of ISU. Similarly, 13th St runs across the northern edges of the downtown area and the university campus. Main north-south roads include Duff Ave and Grand Ave which together carry U.S. Hwy 69 through Ames, University Boulevard and North and South Dakota Avenues.
Ames is very bicycle friendly, with a moderately extensive paved trail system (http://www.cityofames.org...) for walking and biking. Apart from ISU itself, there are generally three main pedestrian-friendly concentrations of shops and restaurants: Downtown on Main Street between Clark and Duff Avenues, Campustown south of Lincoln Way along either side of Welch Ave, and the much newer Somerset neighborhood to the east of Stange Ave north of 24th St. Not quite so pedestrian friendly but with new sidewalks is the two miles of big box stores and strip malls along Duff Avenue between US 30 on the south and Lincoln Way on the north.